The Beckman Coulter CESI 8000 is based on a new front-end separation-and ionization technology called CESI, which fully integrates the high efficiency and ultra-low flow characteristics of capillary electrophoresis (CE) with electrospray ionization (ESI) in a single dynamic process within the same device. Reduction of flow with ESI into the low nanoflow region serves to significantly improve ionization efficiency as well as reduce ion suppression of co-migrating analytes. With CESI we are able to generate stable and robust electrospray with bulk flows of electrolyte at or below 10 nl/min, that when combined with the orthogonal selectivity provided by CE allows for increased coverage and sensitivity.

CESI 8000 with OptiMS Technology provides major advantages over other front-end separations to ESI-MS:

• Increased Sensitivity  – In order of magnitudes depending on analyte and MS
• High Efficiency Separation  – Peak capacity >300
• Decreased Cycle Time  – Short analysis and regeneration times
• Reduced Ion Suppression  – Nanoflow leads to higher sensitivity
• Minute Sampling  – From nano-litre to micro-litre sample introduction
• Broader Analyte Coverage  – Ideal for highly charged and polar analytes